Inflammatory response of strontium titanate micro/nanoparticles in vitro
LIU Wentao1, WU Hong1, ZHOU Jixiang2
1. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China; 2. Xiangya Hospital, Central South University, Changsha 410083, China
Abstract：The biological response of RAW 264.7 cells stimulated by strontium titanate micro/nanoparticles was studied by the direct interaction between cells and materials. Scanning electron microscope (SEM), laser particle size analyzer, X-ray diffractometer and specific surface area tester were used to characterize the physical and chemical properties of the materials. The effects of strontium titanate micro/nanoparticles on the proliferation activity of RAW 264.7 cells were evaluated by CCK-8 test and live/dead cell staining; the morphological changes of cells were observed by SEM; The expression of inflammatory cytokine genes in RAW 264.7 cells stimulated by strontium acid micro/nanoparticles. The results show that the compositions of the micron and nano strontium titanate particles are uniform and both have irregular shapes. The average particle size of the micron strontium titanate particles is 1 085.0 nm, the specific surface area is 1.72 m2/g, and the particle size of the nano strontium titanate particles is 505.2 nm. The specific surface area is 2.94 m2/g. The micron and nano strontium titanate particles have no effect on the proliferation activity of RAW 264.7 cells on the 24h and 72h. Under the stimulation of micro/nano strontium titanate particles, the RAW 264.7 cell morphology develops into a polygonal shape and has a larger attachment area. Micron strontium titanate particles stimulated high expression of inducible nitric oxide synthase (iNOS), cluster of differentiation (CD) 86, TNF-α and interleukin (IL)-6 genes in RAW 264.7 cells, and the expression of iNOS, tumor necrosis factor (TNF)-α and IL-6 genes is significantly higher than that of nano strontium titanate particles. nano strontium titanate particles stimulated RAW 264.7 cells to highly express iNOS and TNF-α genes, while IL-10 gene expression is significantly down-regulated. Both micron and nano strontium titanate particles stimulate RAW 264.7 cells to highly express iNOS, induce RAW 264.7 cells to polarize to the M1 phenotype, and produce an inflammatory response. The inflammatory response induced by micron strontium titanate particles is more significant.
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2021, Vol. 26 
