1. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China; 2. National Key Laboratory of Science and Technology for High-strength Structural Materials, Central South University, Changsha 410083, China
Abstract：Four single-phase carbon materials of resin carbon, carbon fiber, pyrolysis carbon and graphite were studied on the particle shedding and biocompatibility of the materials in simulated body fluids. The amount of particles falling off the surface of materials were studied by using simulated body fluid shock immersion method. The biocompatibility of materials were studied by MTT assay and direct contact experiment. The growth morphology of cells were observed by an inverted biological microscope. The results show that the amount of particles shedding on the surface of the four materials reaches the peak value after soaking in simulated body fluid for 72 h. With the continuous increasing soaking time, the shedding amount of particles decreases and tends to be stable. The most of accumulated shedding amount is graphite for 3.01 mg in the 168 h and the least one is resin carbon for 2.23 mg. The relative growth rates of cells in contact with the four kinds of single-phase carbon material extracts are all greater than 75% within 168 hours, and the cytotoxicity are all for grade 1. The four single-phase carbon materials can inhibit the growth and proliferation of cells, and the degree of inhibition is graphite, pyrolytic carbon, carbon fiber and resin carbon in descending order. The toxicity mechanism of single-phase carbon materials to cells is that the entry of carbon particles into the cell causes the degradation of mitochondria, which can destroy the energy station of the cell, and damage the cell’s DNA. It causes the cell to grow abnormally and even died.
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2021, Vol. 26 
