Abstract Ti, Nb, Zr, Sn, Cu, Ag and Co powders were used as the raw materials, Ti-24Nb-4Zr-7.9Sn(TNZS), TNZS-5Cu, TNZS-5Ag and TNZS-5Co (mass fraction, %) alloys were prepared by high-energy ball milling, cold pressing and vacuum sintering. SD rat osteoblasts were inoculated in dulbecco’s modified eagle medium (DMEM) with 10% fetal calf serum (FBS) and subcultured, and the cell proliferation, cell adhesion and cytotoxicity experiments were carried out. The results showed that addition of 5% Cu, Ag and Co to TNZS alloys could improve the proliferation of osteoblasts to a certain extent. The surface of TNZS-5Co alloy was the most favorable to promote cell adhesion at the early stage of culture (24 h and 72 h). At the later stage of culture (120 h and 168 h), TNZS-5Cu and TNZS-5Ag alloys were more favorable for the adhesion of osteoblasts. The morphology and elongation of osteoblasts attached to the surface of TNZS-5Ag were the best, followed by TNZS-5Co, while the cell adhesion ability of TNZS and TNZS-5Cu were relatively weak. The cytotoxicity grade of the four materials was level 2, which showed that all of them met the cytotoxicity requirements of the biomedical implant materials.
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2020, Vol. 25 
