TNZS基生物材料的体外组织相容性

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Abstract Ti, Nb, Zr, Sn, Cu, Ag and Co powders were used as the raw materials, Ti-24Nb-4Zr-7.9Sn(TNZS), TNZS-5Cu, TNZS-5Ag and TNZS-5Co (mass fraction, %) alloys were prepared by high-energy ball milling, cold pressing and vacuum sintering. SD rat osteoblasts were inoculated in dulbecco’s modified eagle medium (DMEM) with 10% fetal calf serum (FBS) and subcultured, and the cell proliferation, cell adhesion and cytotoxicity experiments were carried out. The results showed that addition of 5% Cu, Ag and Co to TNZS alloys could improve the proliferation of osteoblasts to a certain extent. The surface of TNZS-5Co alloy was the most favorable to promote cell adhesion at the early stage of culture (24 h and 72 h). At the later stage of culture (120 h and 168 h), TNZS-5Cu and TNZS-5Ag alloys were more favorable for the adhesion of osteoblasts. The morphology and elongation of osteoblasts attached to the surface of TNZS-5Ag were the best, followed by TNZS-5Co, while the cell adhesion ability of TNZS and TNZS-5Cu were relatively weak. The cytotoxicity grade of the four materials was level 2, which showed that all of them met the cytotoxicity requirements of the biomedical implant materials.

Key words： TNZS alloy biomedical materials powder metallurgy tissue performance

Received: 20 April 2020 Published: 18 September 2020

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ZHAO Qian. In vitro histocompatibility of TNZS-based biomaterials[J]. Materials Science and Engineering of Powder Metallurgy, 2020, 25(4): 352-357.

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http://pmbjb.csu.edu.cn/EN/ OR http://pmbjb.csu.edu.cn/EN/Y2020/V25/I4/352

[1] 郝玉琳, 杨锐. 纳米高强Ti-Nb-Zr-Sn合金[J]. 金属学报, 2005, 41(11): 25-28.
HAO Yulin, YANG Rui.Nano-high strength Ti-24Nb-4Zr-7.9Sn alloy[J]. Acta Metallurgica Sinica, 2005, 41(11): 25-28.
[2] 杨锐, 郝玉琳. 高强度低模量医用钛合金Ti2448的研制与应用[J]. 新材料产业, 2009, 6(5): 10-13.
YANG Rui, HAO Yulin.Development and application of high strength and low modulus medical titanium alloy Ti2448[J]. Advanced Materials Industry, 2009, 6(5): 10-13.
[3] MUNIR A Z, ANSELMI Y U, OHYANAGI M.The effect of electric field and pressure on synthesis and consolidation of materials: A review of the spark plasma sintering method[J]. Journal of Materials Science, 2006, 41(3): 763-777.
[4] 于振涛, 余森, 张明华, 等. 外科植入物用新型医用钛合金材料设计、开发与应用现状及进展[J]. 中国材料进展, 2010, 29(12): 35-51.
YU Zhentao, YU Sen, ZHANG Minghua, et al.Current status and progress of design, development and application of new medical titanium alloy materials for surgical implants[J]. Materials China, 2010, 29(12): 35-51.
[5] 徐丽娟. 无毒生物医用β型钛合金材料凝固组织和性能的研究[D]. 哈尔滨: 哈尔滨工业大学, 2003.
XU LiJuan.Study on solidification structure and properties of non-toxic biomedical titanium alloy[D]. Harbin: Harbin Institute of Technology, 2003.
[6] 于洋, 刘伟. 几种含铌低弹性模量钛合金组织与性能研究[J]. 稀有金属, 2004, 28(2): 419-420.
YU Yang, LIU Wei.Microstructure and properties of several titanium alloys with low elastic modulus of niobium[J]. Rare Metals, 2004, 28(2): 419-420.
[7] 于振涛, 余森, 程军, 等. 新型医用钛合金材料的研发和应用现状[J]. 金属学报, 2017, 53(10): 1238-1264.
YU Zhentao, YU Sen, CHENG Jun, et al.Development and application of new medical titanium alloy material[J]. Acta Metallurgica Sinica, 2017, 53(10): 1238-1264.
[8] 王紫琴. Ag、Cu离子注入医用金属材料表面改性研究[D]. 天津: 天津大学, 2006.
WANG Ziqin.Study on surface modification of Ag and Cu ion implanted medical metal materials[D]. Tianjin: Tianjin University, 2006.
[9] MOLL J H, MCT IERNAN B J. PM TiAl alloys: the sky’s the limit[J]. Metal Powder Report, 2000, 55(1): 18-22.
[10] YOLTON C F, KIM Y W, HABEL U.Powder metallurgy processing of gamma titanium aluminide[C]// ROSENBERGER A H. 2004 TMS Annual Meeting: 3rd International Symposium on Gamma Titanium Aluminides (ISGTA 2003). Warrendale P A, USA: TMS, 2003: 233-240.
[11] 柳正明. 经微弧氧化表面处理的钛铌锆锡合金的生物相容性研究[D]. 西安: 第四军医大学, 2008.
LIU Zhengming.Study on biocompatibility TNZS alloy treated by micro-arc oxidation[D]. Xi’an: The Fourth Military Medical University, 2008.
[12] 姚孟宇. 新型抗菌钛合金Ti6Al4V5Cu的生物相容性及成骨性能研究[D]. 上海: 第二军医大学, 2016.
YAO Mengyu.Study on biocompatibility and osteogenesis of the new antibacterial titanium alloy Ti6Al4V5Cu[D]. Shanghai: The Second Military Medical University, 2016.
[13] 陈良建, 张思慧, 李益民,等. 改性后不同孔隙度多孔钛对成骨细胞的影响[J]. 中国有色金属学报, 2010, 20(4): 749-755.
CHEN Liangjian, ZHANG Sihui, LI Yimin, et al.Effects of modified porous titanium with different porosity on osteoblasts[J]. Chinese Journal of Nonferrous Metals, 2010, 20(4): 749-755.
[14] 赵倩. 高能球磨冷压烧结制备Ti-24Nb-4Zr-7.9Sn-xMe(Me= Cu,Ag,Co)生物医用材料的组织与性能研究[D]. 镇江: 江苏大学, 2018.
ZHAO Qian.Study on microstructure and properties of biomedical Ti-24Nb-4Zr-7.9Sn-xMe(Me=Cu,Ag,Co) prepared by high-energy milling and cold pressed sintering[D]. Zhenjiang: Jiangsu University, 2018.